Journal: The EMBO Journal

Article Title: Elongator is a microtubule polymerase selective for polyglutamylated tubulin

doi: 10.1038/s44318-024-00358-0

Figure Lengend Snippet: Reagents and tools table

Article Snippet: AMCA-Porcine tubulin , Cytoskeleton , TL440M.

Techniques: Recombinant, Sequencing, Protease Inhibitor, Software

Journal: Nature Biotechnology

Article Title: Directed evolution and selection of biostable l -DNA aptamers with a mirror-image DNA polymerase

doi: 10.1038/s41587-022-01337-8

Figure Lengend Snippet: a , Schematic overview of detecting native human thrombin with the l -DNA aptamer sensor, by hybridizing the l -9-1t aptamer strand (cyan) with an l -DNA fluorophore strand labeled with FAM (green) and an l -DNA quencher strand labeled with DABCYL (red). b , Measured relative fluorescence of the l -DNA aptamer sensor incubated with 1 μM native human thrombin in physiological buffer alone, or physiological buffer with 10% human serum for up to 48 min, with excitation wavelength at 494 nm and emission wavelength at 518 nm, and measurements taken every 4 min. NC1, negative control with the l -DNA aptamer sensor in physiological buffer alone. NC2, negative control with the l -DNA aptamer sensor in physiological buffer with 10% human serum. RFU, relative fluorescence unit. Data are presented as mean ± s.d. ( n = 3, independent measurements). c , Measured thrombin concentrations by the d - and l -DNA aptamer sensors with 300 nM native human thrombin in physiological buffer alone, or in physiological buffer with 10% human serum or 50 units ml -1 natural DNase I, incubated for up to 4 h. Data are presented as mean ± s.d. ( n = 3, independent measurements), with the corresponding data shown in Supplementary Table . d , Schematic overview of detecting native human thrombin immobilized on a nitrocellulose membrane with l -DNA aptamer western blot. e – g , Native human thrombin ( e and g ) or streptavidin ( f ) separated by 15% SDS–PAGE, transferred to a nitrocellulose membrane, incubated with 500 nM Cy5- l -13t aptamer ( e and f ) or mouse monoclonal primary antibody targeting native human thrombin and Alexa Fluor 647-labeled goat anti-mouse IgG polyclonal secondary antibody ( g ) and scanned by the Amersham Typhoon Biomolecular Imager under the Cy5 mode. M, prestained protein marker labeled with fluorescent dye. The experiments were performed twice with similar results.

Article Snippet: Prestained protein marker labeled with fluorescent dye was purchased from Solarbio Life Sciences.

Techniques: Labeling, Fluorescence, Incubation, Negative Control, Membrane, Western Blot, SDS Page, Marker